Development of Enzyme-Based Cosmeceuticals: Studies on the Proteolytic Activity of Arthrospira platensis and Its Efficient Incorporation in a Hydrogel Formulation

Microalgae are a valuable source of enzymes and active ingredients due to their biochemical variability, health-beneficial properties, lower production cost viability on an industrial scale. In addition, the growing demand for sustainable products constitutes microalgae as viable resource development range innovative products. present work, proteolytic activity Arthrospira platensis lysate was assessed characterized using kinetics analysis zymography employing different substrates, pH values divalent ions. The results suggest presence two main in lysate. rapid easy purification protocol activity, under mild conditions, developed polyethyleneglycol (PEG)/phosphate aqueous two-phase system (ATPS). A. used develop hydrogel formulation enzyme-based cosmeceutical, with potential application topical exfoliating agent. incorporation extract significantly improved its operational stability over time, which is significant advantage product development.